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Composite

Part:BBa_M1529:Design

Designed by: Sarah Allred   Group: UtahState_BE5930_-_S11   (2012-04-17)


Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 923
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4768
    Illegal SpeI site found at 923
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2241
    Illegal BglII site found at 2904
    Illegal BglII site found at 6798
    Illegal BglII site found at 7461
    Illegal BamHI site found at 5287
    Illegal XhoI site found at 5188
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 923
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 923
    Illegal NgoMIV site found at 1171
    Illegal NgoMIV site found at 5728
    Illegal AgeI site found at 658
    Illegal AgeI site found at 4410
    Illegal AgeI site found at 4467
    Illegal AgeI site found at 8967
    Illegal AgeI site found at 9024
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See subparts



Source

See subparts


References